Planar cell polarity-mediated induction of neural stem cell
نویسندگان
چکیده
32 Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the 33 spinal cord. How a neural stem cell under homeostasis converts after injury to a highly 34 regenerative cell remains unknown. Here we show that during regeneration, axolotl neural stem 35 cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic 36 neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the 37 switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity 38 (PCP) pathway components. We show that PCP induction is essential to reorient mitotic 39 spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal 40 axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our 41 findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth 42 with the switch from a homeostatic to a regenerative stem cell that restores missing tissue. 43 3
منابع مشابه
Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. ...
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